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KMID : 0613820090190050676
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Journal of Life Science
2009 Volume.19 No. 5 p.676 ~ p.679
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Anti-cancer Activity of Supernatant of Rahnella aquatilis AY 2000 Cocultured with Streptomyces griseus
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Kim Ji-Hyun
Kim Kwang-Hyeon
Lee Jong-Hwan
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Abstract
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In order to produce a new antibiotic material against Jurkat T cells using horizontal gene transfer among microbes, co-cultures between soil bacteria AY2000 and the multiple antibiotic producer S. griseus was carried out. It showed that the highest active substance against Jurkat T cells was produced at 48 hr of co-culture time with MTT assay. Moreover, a morphological change of nuclear of Jurkat T cells treated with co-cultured substance was observed in DAPI staining. This result suggests that a new material was produced with co-culture supernatant, and that co-culture between microboes can develop new antibiotic materials.
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KEYWORD
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Coculture, horizontal gene transfer, AY2000, S. griseus, antibiotic material
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